Monday, 09 December 2019
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EURL STANDARD OPERATING PROCEDURES (SOP) FOR ASF DIAGNOSIS

 

  • Sampling processing for ASFV diagnosis. Download (PDF).

 

ASF ANTIBODY DETECTION. 

PRODUCTION OF THE ASFV ANTIBODY REAGENTS.

  • Obtention of the ASFV cytoplasmic soluble antigen for using on the OIE-antibody detction tests. Download (PDF)
  • Production of the OIE-Immunoblotting (IB) strips for ASF antibody detection. Download (PDF)
  • Preparation of ASFV-coated 96-well plates for the OIE-Indirect immunoperoxidase test (IPT). Download (PDF)

OIE-ASFV ANTIBODY DETECTION TESTs  

  • Detection of ASFV antibodies by indirect ELISA. Download (PDF).
  • Detection of ASFV antibodies by Immunoblotting. Download (PDF).
  • Detection of ASFV antibodies  Indirect Immunoperoxidase test (IPT). Download (PDF)

ASF VIRUS DETECTION

  • ASF virus isolation using porcine leukocytes. Download (PDF).
  • ASF virus isolation using porcine alveolar macrophages. Download (PDF).
  • Extraction of ASFV DNA. Download (PDF).
  • Detection of ASFV by the OIE Real-Time PCR. Download (PDF).
  • Detection of ASFV by the UPL Real-Time PCR. Download (PDF).
  • Detection of ASFV by conventional PCR. Download (PDF).

GENOTYPING OF ASFV ISOLATES.

  • ASFV Genotyping. Download (PDF).

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