EURL STANDARD OPERATING PROCEDURES (SOP) FOR ASF DIAGNOSIS

• Sampling processing for ASFV diagnosis. Download (PDF).

ASF ANTIBODY DETECTION. 

PRODUCTION OF THE ASFV ANTIBODY REAGENTS.

• Obtention of the ASFV cytoplasmic soluble antigen for using on the OIE-antibody detction tests. Download (PDF)

• Production of the OIE-Immunoblotting (IB) strips for ASF antibody detection. Download (PDF)

• Preparation of ASFV-coated 96-well plates for the OIE-Indirect immunoperoxidase test (IPT). Download (PDF)

OIE-ASFV ANTIBODY DETECTION TESTs  

• Detection of ASFV antibodies by indirect ELISA. Download (PDF).

• Detection of ASFV antibodies by Immunoblotting. Download (PDF).

• Detection of ASFV antibodies  Indirect Immunoperoxidase test (IPT). Download (PDF)

ASF VIRUS DETECTION

• ASF virus isolation using porcine leukocytes. Download (PDF).

• ASF virus isolation using porcine alveolar macrophages. Download (PDF).

• Extraction of ASFV DNA. Download (PDF).

• Detection of ASFV by the OIE Real-Time PCR. Download (PDF).

• Detection of ASFV by the UPL Real-Time PCR. Download (PDF).

• Detection of ASFV by conventional PCR. Download (PDF).

GENOTYPING OF ASFV ISOLATES.

• ASFV Genotyping. Download (PDF).