Publications

2024

2023

2022

2021

2020

Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay

Aira C, González-García G, Martínez-Cano J, de la Roja N, Giammarioli M, Feliziani F, Šteingolde Ž, Buitkuviene J, Václavek P, Glišić D, Gallardo C, Sastre P, García-Durán M, Rueda P, Fresco-Taboada A.

Vaccines (Basel). 2024 Mar 14;12(3):307. doi: 10.3390/vaccines12030307.

Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1

Pedrera M, Soler A, Simón A, Casado N, Pérez C, García-Casado MA, Fernández-Pacheco P, Sánchez-Cordón PJ, Arias M, Gallardo C.

Vaccines (Basel). 2024 Apr 20;12(4):443. doi: 10.3390/vaccines12040443.

	The Production of Recombinant African Swine Fever Virus Lv17/WB/Rie1 Strains and Their In Vitro and In Vivo Characterizations Petrini S, Righi C, Mészáros I, D‘Errico F, Tamás V, Pela M, Olasz F, Gallardo C, Fernandez-Pinero J, Göltl E, Magyar T, Feliziani F, Zádori Z. Vaccines (Basel). 2023 Dec 17;11(12):1860. doi: 10.3390/vaccines1112186.
	Improving African Swine Fever Surveillance Using Fluorescent Rapid Tests Aira C, Monedero A, Hernández-Antón S, Martínez-Cano J, Camuñas A, Casado N, Nieto R, Gallardo C, García-Durán M, Rueda P, Fresco-Taboada A. Pathogens. 2023 Jun 7;12(6):811. doi: 10.3390/pathogens12060811. PMID: 37375501; PMCID: PMC10303979.
	Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia Glišić D, Milićević V, Krnjaić D, Toplak I, Prodanović R, Gallardo C, Radojičić S. Vet Res Commun. 2023 May 31. doi: 10.1007/s11259-023-10145-7. Epub ahead of print. PMID: 37256519.
	A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African Swine Fever viruses circulating from 2007 to 2022 Gallardo C, Casado N, Soler A, Djadjovski I, Krivko L, Madueño E, Nieto R, Perez C, Simon A, Ivanova E, Donescu D, Milicevik V, Chondrokouki E, Nurmoja I, Frant M, Feliziani F, Václavek P, Pileviciene S, Arias M. Vet Res Commun. 2023 May 31. doi: 10.1007/s11259-023-10145-7. Epub ahead of print. PMID: 37256519.

Indirect Immunoperoxidase Test (IPT) for Detection of Antibodies Against African Swine Fever Virus (ASFV) on African Green Monkey Cell Lines (Vero, MS)

Carmina G, Nieto R, Arias M.

Methods Mol Biol. 2022;2503:147-158. doi: 10.1007/978-1-0716-2333-6_10. PMID: 35575892.

African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Semi- Purified Antigen (ASFV CP-Ag)

Carmina G, Nieto R, Arias M.

Methods Mol Biol. 2022;2503:133-145. doi: 10.1007/978-1-0716-2333-6_9. PMID: 35575891.

Dynamics of African Swine Fever virus (ASFV) infection in domestic pigs infected with virulent, moderate virulent and attenuated genotype II ASFV European isolates

Gallardo C, Soler A, Nurmoja I, Cano-Gómez C, Cvetkova S, Frant M, Woźniakowski G, Simón A, Pérez C, Nieto R, Arias M.

Transbound Emerg Dis. 2021 Jul 17. doi: 10.1111/tbed.14222. Epub ahead of print. PMID: 34273247.

Effect of mixing and feed batch sequencing on the prevalence and distribution of African swine fever virus in swine feed

Elijah CG, Trujillo JD, Jones CK, Kwon T, Stark CR, Cool KR, Paulk CB, Gaudreault NN, Woodworth JC, Morozov I, Gallardo C, Gebhardt JT, Richt JA.

Transbound Emerg Dis. 2021 Jun 2. doi: 10.1111/tbed.14177. Epub ahead of print. PMID: 34076951.

High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge

Cadenas-Fernández E, Sánchez-Vizcaíno JM, van den Born E, Kosowska A, van Kilsdonk E, Fernández-Pacheco P, Gallardo C, Arias M, Barasona JA.

Vaccines (Basel). 2021 Mar 10;9(3):242. doi: 10.3390/vaccines9030242. PMID: 33802021; PMCID: PMC7999564.

Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection

Lopez E, van Heerden J, Bosch-Camós L, Accensi F, Navas MJ, López-Monteagudo P, Argilaguet J, Gallardo C, Pina-Pedrero S, Salas ML, Salt J, Rodriguez F.

Viruses. 2020 Dec 21;12(12):1474. doi: 10.3390/v12121474. PMID: 33371460; PMCID: PMC7767464.

Identification and Isolation of Two Different Subpopulations Within African Swine Fever Virus Arm/07 Stock

Pérez-Núñez D, Castillo-Rosa E, Vigara-Astillero G, García-Belmonte R, Gallardo C, Revilla Y.

Vaccines (Basel). 2020 Oct 25;8(4):625. doi: 10.3390/vaccines8040625. PMID: 33113838; PMCID: PMC7712101.

Molecular Characterization of African Swine Fever Virus Isolates in Estonia in 2014-2019

Vilem A, Nurmoja I, Niine T, Riit T, Nieto R, Viltrop A, Gallardo C.

Pathogens. 2020 Jul 17;9(7):582. doi: 10.3390/pathogens9070582. PMID: 32709020; PMCID: PMC 7400522.

2019

2018

2017

2016

2015

	African Swine Fever (ASF) diagnosis, an essential tool in the epidemiological investigation Gallardo C, Fernández-Pinero J, Arias M. Virus Res. 2019 Oct 2;271:197676. doi: 10.1016/j.virusres.2019.197676. Epub 2019 Jul 27. Review. PubMed PMID: 31362027.
	Identification and Immunogenicity of African Swine Fever Virus Antigens Gallardo C, Fernández-Pinero J, Arias M. Front Immunol. 2019 Jun 19;10:1318. doi: 10.3389/fimmu.2019.01318. eCollection 2019. PubMed PMID: 31275307; PubMed Central PMCID: PMC6593957.
	First Oral Vaccination of Eurasian Wild Boar Against African Swine Fever Virus Genotype II Gallardo C, Nurmoja I, Barasona JA, Gallardo C, Cadenas-Fernández E, Jurado C, Rivera B, Rodríguez-Bertos A, Arias M, Sánchez-Vizcaíno JM. Front Vet Sci. 2019 Apr 26;6:137. doi: 10.3389/fvets.2019.00137.
	Genetic characterization of African Swine Fever virus in Cameroon, 2010-2018 Wade A, Achenbach JE, Gallardo C, Settypalli TBK, Souley A, Djonwe G, Loitsch A, Dauphin G, Ngang JJE, Boyomo O, Cattoli G, Diallo A, Lamien CE. J Microbiol. 2019 Apr;57(4):316-324. doi: 10.1007/s12275-019-8457-4. Epub 2019 Mar 30.
	Attenuated and non-haemadsorbing (non-HAD) genotype II African swine fever virus (ASFV) isolated in Europe, Latvia 2017 Gallardo C, Soler A, Rodze I, Nieto R, Cano-Gómez C, Fernandez-Pinero J, Arias M. Transbound Emerg Dis. 2019 May;66(3):1399-1404. doi: 10.1111/tbed.13132. Epub 2019 Feb 18. PubMed PMID:30667598.

Evolution in Europe of African swine fever genotype II viruses from highly to moderately virulent

Gallardo C, Nurmoja I, Soler A, Delicado V, Simón A, Martin E, Perez C, Nieto R, Arias M.

Veterinary Microbiology. 219. 10.1016/j.vetmic.2018.04.001.

African Swine Fever virus (ASFV) protection mediated by NH/P68 and NH/P68 recombinant live-attenuated viruses

Gallardo C, Sánchez EG, Pérez-Núñez D, Nogal M, de León P, Carrascosa ÁL, Nieto R, Soler A, Arias ML, Revilla Y.

Vaccine. 2018 May 3;36(19):2694-2704pii: S0264-410X (18)30385-2. doi: 10.1016/j.vaccine.2018.03.040.

Phylodynamics and evolutionary epidemiology of African Swine Fever p72-CVR genes in Eurasia and Africa

Alkhamis MA, Gallardo C, Jurado C, Soler A, Arias M, Sánchez-Vizcaíno JM.

PLoS One. 2018 Feb 28;13(2). Review.

Transmission of African Swine Fever virus from infected pigs by direct contact and aerosol routes

Olesen AS, Lohse L, Boklund A, Halasa T, Gallardo C, Pejsak Z, Belsham GJ, Rasmussen TB, Bøtner A.

Vet Microbiol. 2017 Nov;211:92-102. doi: 10.1016/j.vetmic.2017.10.004. Adjuntar publi

Epub 2017 Oct 4. PubMed PMID: 29102127.

Approaches and Perspectives for Development of African Swine Fever Virus Vaccines

Arias M, De la Torre A, Dixon L, Gallardo C, Jori F, Laddomada A, Martins C, Parkhouse RM, Revilla Y, Rodriguez FAJ; Sanchez-Vizcaino.

Vaccines (Basel). 2017 Oct 7;5(4). pii: E35. doi: 10.3390/vaccines5040035. Review.

Gaps in African swine fever: Analysis and priorities

Arias M, Jurado C, Gallardo C, Fernández-Pinero J, Sánchez-Vizcaíno JM.

Transbound Emerg Dis. 2017 Sep 22.

Phenotyping and susceptibility of established porcine cells lines to African Swine Fever Virus infection and viral production

Sánchez EG, Riera E, Nogal M, Gallardo C, Fernández P, Bello-Morales R, López-Guerrero JA, Chitko-McKown CG, Richt JA, Revilla Y.

Sci Rep. 2017 Sep 4;7(1):10369.

	Development of a novel lateral flow assay for detection of African Swine Fever in blood Sastre P, Gallardo C, Monedero A, Ruiz T, Arias M, Sanz A, Rueda P. BMC Vet Res. 2016 Sep 15;12:206. doi: 10.1186/s12917-016-0831-4. PubMed PMID: 27633968.
	Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA Giménez-Lirola LG, Mur L, Rivera B, Mogler M, Sun Y, Lizano S, Goodell C, Harris DL, Rowland RR, Gallardo C, Sánchez-Vizcaíno JM, Zimmerman J. PLoS One. 2016 Sep 9;11(9).
	Development of a duplex lateral flow assay for simultaneous detection of antibodies against African and Classical Swine Fever viruses Sastre P, Pérez T, Costa S, Yang X, Räber A, Blome S, Goller KV, Gallardo C, Tapia I, García J, Sanz A, Rueda P. J Vet Diagn Invest. 2016 Sep;28(5):543-9.
	Identification of a New Genotype of African Swine Fever Virus in Domestic Pigs from Ethiopia Achenbach JE, Gallardo C, Nieto-Pelegrín E, Rivera-Arroyo B, Degefa-Negi T, Arias M, Jenberie S, Mulisa DD, Gizaw D, Gelaye E, Chibssa TR, Belaye A, Loitsch A, Forsa M, Yami M, Diallo A, Soler A, Lamien CE, Sánchez-Vizcaíno JM. Transbound Emerg Dis. 2016 May 22.
	African Swine Fever virus introduction into the EU in 2014: Experience of Latvia Oļševskis E, Guberti V, Seržants M, Westergaard J, Gallardo C, Rodze I, Depner K. Res Vet Sci. 2016 Apr;105:28-30.
	African Swine Fever virus introduction into the EU in 2014: Experience of Latvia Mª Carmen Gallardo, Ana de la Torre Reoyo, Jovita Fernández-Pinero, Irene Iglesias, Mª Jesús Muñoz, and Mª Luisa Arias. Porcine Health Management (2015) 1:21DOI 10.1186/s40813-015-0013-y.

	Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs C Gallardo, A Soler, R Nieto, MA Sánchez, C Martins, V Pelayo, A Carrascosa, Y Revilla, A Simón, V Briones, JM Sánchez-Vizcaíno, M Arias. Transbound Emerg Dis. 2015 Dec;62(6):612-22.
	Experimental Infection of Domestic Pigs with African Swine Fever Virus Lithuania 2014 Genotype II Field Isolate Gallardo C, Soler A, Nieto R, Cano C, Pelayo V, Sánchez MA, Pridotkas G, Fernandez-Pinero J, Briones V, Arias M. Transbound Emerg Dis. 2015 Mar 22.
	Assessment of African Swine Fever Diagnostic Techniques as a Response to the Epidemic Outbreaks in Eastern European Union Countries: How to Improve Surveillance and Control Programmes Gallardo C, Nieto R, Soler A, Pelayo V, Fernández-Pinero J, Markowska-DanielI, Pridotkas G, Nurmoja I, Granta R, Simón A, Pérez C, Martín E, Fernández-Pacheco P, Arias M. J Clin Microbiol. 2015 Jun 3.
	Comparative analysis of the complete genome sequences of Kenyan African Swine Fever virus isolates within p72 genotypes IX and X Bishop RP, Fleischauer C, De Villiers EP, Okoth EA, Arias M, Gallardo C, Upton C. Virus Genes. 2015 Feb 3.

2014

2013

2012

2011

2010

African Swine Fever Diagnosis Adapted to Tropical Conditions by the Use of Dried-blood Filter Papers

T. Randriamparany, K. V. Kouakou, V. Michaud, J. Fernandez-Pinero, C. Gallardo, M.-F. Le Potier, R. Rabenarivahiny, E. Couacy-Hymann, M. Raherimandimby and E. Albina.

Transbound Emerg Dis. 2014 Nov 27.

Genetic Variation among African Swine Fever Genotype II Viruses, Eastern and Central Europe

Carmina Gallardo, Jovita Fernández-Pinero, Virginia Pelayo, Ismail Gazaev, Iwona Markowska-Daniel, Gediminas Pridotkas, Raquel Nieto, Paloma Fernández-Pacheco, Svetlana Bokhan, Oleg Nevolko, Zhanna Drozhzhe, Covadonga Pérez, Alejandro Soler, Denis Kolvasov, and Marisa Arias.

Emerging infectious Diseases. Volume 20, Number 9—September 2014.

	Potential use of oral fluid samples for serological diagnosis of African Swine Fever Lina Mur, Carmina Gallardo, Alejandro Soler, Jeffrey Zimmermman, Virginia Pelayo, Raquel Nieto, Jose Manuel Sanchez-Vizcaino, Marisa Arias. Veterinary Microbiology 165 (2013) 135–139.
	Molecular diagnosis of African Swine Fever by a new real-time PCR using universal probe library Fernández-Pinero J, Gallardo C, Elizalde M, Robles A, Gómez C, Bishop R, Heath L, Couacy-Hymann E, Fasina FO, Pelayo V, Soler A, Arias M. Transbound Emerg Dis. 2013 Feb;60(1):48-58.
	Comparative evaluation of novel African Swine Fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests Gallardo C, Soler A, Nieto R, Carrascosa AL, De Mia GM, Bishop RP, Martins C, Fasina FO, Couacy-Hymman E, Heath L, Pelayo V, Martín E, Simón A, Martín R, Okurut AR, Lekolol I, Okoth E, Arias M. Vet Microbiol. 2013 Feb 22;162(1):32-43.

	DNA vaccination partially protects against African Swine Fever virus lethal challenge in the absence of antibodies Argilaguet JM, Pérez-Martín E, Nofrarías M, Gallardo C, Accensi F, Lacasta A, Mora M, Ballester M, Galindo-Cardiel I, López-Soria S, Escribano JM, Reche PA, Rodríguez F. PLoS One. 2012;7(9).
	Risk factors for farm-level African Swine Fever infection in major pig-producing areas in Nigeria, 1997-2011 Fasina FO, Agbaje M, Ajani FL, Talabi OA, Lazarus DD, Gallardo C, Thompson PN, Bastos AD. Prev Vet Med. 2012 Nov 1;107(1-2):65-75.
	Development of a Suspension Microarray for the Genotyping of African Swine Fever Virus Targeting the SNPs in the C-Terminal End of the p72 Gene Region of the Genome Leblanc N, Cortey M, Fernandez Pinero J, Gallardo C, Masembe C, Okurut AR, Heath L, van Heerden J, Sánchez-Vizcaino JM, Ståhl K, Belák S. Transbound Emerg Dis. 2012 Jul 8.
	Molecular characterization of African Swine Fever virus isolates originating from outbreaks in the Russian Federation between 2007 and 2011 Malogolovkin A, Yelsukova A, Gallardo C, Tsybanov S, Kolbasov D. Vet Microbiol. 2012 Aug 17;158(3-4):415-9.
	Monitoring of African Swine Fever in the wild boar population of the most recent endemic area of Spain Mur L, Boadella M, Martínez-López B, Gallardo C, Gortazar C, Sánchez-Vizcaíno JM. Transbound Emerg Dis. 2012 Dec;59(6):526-31.
	Comparison of African Swine Fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya Okoth E, Gallardo C, Macharia JM, Omore A, Pelayo V, Bulimo DW, Arias M, Kitala P, Baboon K, Lekolol I, Mijele D, Bishop RP. Prev Vet Med. 2012 Dec 6.

	Enhancing DNA immunization by targeting ASFV antigens to SLA-II bearing cells Argilaguet JM, Pérez-Martín E, Gallardo C, Salguero FJ, Borrego B, Lacasta A, Accensi F, Díaz I, Nofrarías M, Pujols J, Blanco E, Pérez-Filgueira M, Escribano JM, Rodríguez F. Vaccine. 2011 Jul 26;29(33):5379-85.
	Disruption of nuclear organization during the initial phase of African Swine Fever virus infection Ballester M, Rodríguez-Cariño C, Pérez M, Gallardo C, Rodríguez JM, Salas ML, Rodriguez F. J Virol. 2011 Aug;85(16):8263-9.
	African Swine Fever virus p72 genotype IX in domestic pigs, Congo, 2009 Gallardo C, Anchuelo R, Pelayo V, Poudevigne F, Leon T, Nzoussi J, Bishop R, Pérez C, Soler A, Nieto R, Martín H, Arias M. Emerg Infect Dis. 2011 Aug;17(8):1556-8.
	Genotyping of African Swine Fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007 Gallardo C, Ademun AR, Nieto R, Nantima N, Arias M, Martín E, Pelayo V and Bishop Richard P. African Journal of Biotechnology (AJB). April 2011; 10 (17); pp. 3488-3497.
	African Swine Fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site Gallardo C, Okoth E, Pelayo V, Anchuelo R, Martín E, Simón A, Llorente A, Nieto R, Soler A, Martín R, Arias M, Bishop RP. J Gen Virol. 2011Feb;92(Pt 2):432-44.
	Genetic characterisation of African Swine Fever viruses from recent and historical outbreaks in Sardinia (1978-2009) Giammarioli M, Gallardo C, Oggiano A, Iscaro C, Nieto R, Pellegrini C, Dei Giudici S, Arias M, De Mia GM. Virus Genes. 2011 Jun;42(3):377-87.
	Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African Swine Fever virus Tignon M, Gallardo C, Iscaro C, Hutet E, Van der Stede Y, Kolbasov D, De Mia GM, Le Potier MF, Bishop RP, Arias M, Koenen F. J Virol Methods. 2011 Dec;178(1-2):161-70.
	Design and verification of a highly reliable Linear-After-The-Exponential PCR (LATE-PCR) assay for the detection of African Swine Fever virus Ronish B, Hakhverdyan M, Ståhl K, Gallardo C, Fernandez-Pinero J, Belák S, Leblanc N, Wangh L. J Virol Methods. 2011 Mar;172(1-2):8-15. doi: 10.1016/j.jviromet.2010.12.003. Epub 2010 Dec 15. Erratum in: J Virol Methods. 2011 May;173(2):403. Gallardo, C [added]; Fernandez-Pinero, J [added].

	Intranuclear detection of African Swine Fever virus DNA in several cell types from formalin-fixed and paraffin-embedded tissues using a new in situ hybridisation protocol Ballester M, Galindo-Cardiel I, Gallardo C, Argilaguet JM, Segalés J, Rodríguez JM, Rodríguez F. J Virol Methods. 2010 Sep;168(1-2):38-43.
	Phylogenomic analysis of 11 complete African Swine Fever virus genome sequences De Villiers EP, Gallardo C, Arias M, Da Silva M, Upton C, Martin R, Bishop RP. Virology. 2010 Apr 25;400(1):128-36.
	Surveillance for African Swine Fever in Nigeria, 2006-2009 Fasina FO, Shamaki D, Makinde AA, Lombin LH, Lazarus DD, Rufai SA, Adamu SS, Agom D, Pelayo V, Soler A, Simón A, Adedeji AJ, Yakubu MB, Mantip S, Benshak AJ, Okeke I, Anagor P, Mandeng DC, Akanbi BO, Ajibade AA, Faramade I, Kazeem MM, Enurah LU, Bishop R, Anchuelo R, Martin JH, Gallardo C. Transbound Emerg Dis. 2010 Aug 1;57(4):244-53.
	Sensitive detection of African Swine Fever virus using real-time PCR with a 5′ conjugated minor groove binder probe McKillen J, McMenamy M, Hjertner B, McNeilly F, Uttenthal A, Gallardo C, Adair B, Allan G. J Virol Methods. 2010 Sep;168(1-2):141-6.