EURL Standard Operating Procedures (SOP) for ASF Diagnosis
This section provided detailed standard operating procedures (SOPs) of ASF diagnostic methods validated and currently available at the EURL-ASF. The interpretation of the results is reported in each procedure.
Sampling processing
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Processing of samples for African Swine Fever (ASF) diagnosis SOP/CISA/ASF/SAMPLES/1/. Method accredited UNE-EN ISO/IEC 17025:2017. |
African Swine Fever Virus (ASFV) Genome Detection
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Procedure for the extraction of the ASF virus (ASFV) DNA SOP/CISA/ASF/DNA EXTRACTION/1/. Method accredited UNE-EN ISO/IEC 17025:2017. |
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Detection of the ASFV genome by the WOAH-conventional PCR, Agüero et al., 2003 SOP/CISA/ASF/PCR/1. Method accredited UNE-EN ISO/IEC 17025:2017. |
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Detection of the ASFV genome by the WOAH- real time PCR (1), King et al., 2003 SOP/CISA/ASF/PCR/2. Method accredited UNE-EN ISO/IEC 17025:2017. |
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Detection of the ASFV genome by the WOAH- real time PCR (2), Fernández et al., 2013 (Taqman probe ASFV-VP72P1 or UPL commercial probe) SOP/CISA/ASF/PCR/3. Method accredited UNE-EN ISO/IEC 17025:2017. |
ASF Antibody Detection Techniques
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Detection of ASFV antibodies by the WOAH-indirect ELISA test SOP/CISA/ELISA/1. Method accredited UNE-EN ISO/IEC 17025:2017. |
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Detection of ASFV antibodies by the commercial INGENASA blocking ELISA test (INGEZIM PPA COMPAC K3.) SOP/CISA/ELISA/2. Method accredited UNE-EN ISO/IEC 17025:2017. |
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Detection of ASFV antibodies by the WOAH-Immunoblotting (IB) SOP/CISA/IB/1. Method accredited UNE-EN ISO/IEC 17025:2017. |
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Detection of ASFV antibodies by the WOAH-immunoperoxidase test (IPT) SOP/CISA/IPT/1. Method accredited UNE-EN ISO/IEC 17025:2017. |
Production of the ASFV Antibody Reagents
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SOP/CISA/ASF-Ag/1. |
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Production of the WOAH-Immunoblotting (IB) strips for ASF antibody detection SOP/CISA/ASF/IB/STRIPS/1. |
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Preparation of the ASFV-coated 96-well plates for the WOAH-Indirect immunoperoxidase test (IPT) SOP/CISA/ASF/IPT-PLATES/1. |
Genotyping of ASFV isolates
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Procedure for the genotyping of ASFV isolates by the analysis of partial regions of the ASFV genome SOP/CISA/ASF/GENOTYPING/1. |
ASFV isolation, titration and identification by the haemadsorbing (HAD) test in swine primary cell cultures
ASFV growing and titration on established cell lines
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Procedure for growing and preparation of established cell lines for ASFV propagation (VERO, MS) SOP/CISA/ASF/CELLS/1. |
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Procedure for ASFV growing and titration in established cell lines (VERO, MS) SOP/CISA/ASF/TITRATION/1. |