EURL Standard Operating Procedures (SOP) for ASF Diagnosis

This section provided detailed standard operating procedures (SOPs) of ASF diagnostic methods validated and currently available at the EURL-ASF. The interpretation of the results is reported in each procedure.

Sampling processing

Processing of samples for African Swine Fever (ASF) diagnosis

SOP/CISA/ASF/SAMPLES/1/.

Method accredited UNE-EN ISO/IEC 17025:2017.

African Swine Fever Virus (ASFV) Genome Detection

Procedure for the extraction of the ASF virus (ASFV) DNA

SOP/CISA/ASF/DNA EXTRACTION/1/.

Method accredited UNE-EN ISO/IEC 17025:2017.

Detection of the ASFV genome by the WOAH-conventional PCR, Agüero et al., 2003

SOP/CISA/ASF/PCR/1.

Method accredited UNE-EN ISO/IEC 17025:2017.

Detection of the ASFV genome by the WOAH- real time PCR (1), King et al., 2003

SOP/CISA/ASF/PCR/2.

Method accredited UNE-EN ISO/IEC 17025:2017.

Detection of the ASFV genome by the WOAH- real time PCR (2), Fernández et al., 2013 (Taqman probe ASFV-VP72P1 or UPL commercial probe)

SOP/CISA/ASF/PCR/3.

Method accredited UNE-EN ISO/IEC 17025:2017.

ASF Antibody Detection Techniques

Detection of ASFV antibodies by the WOAH-indirect ELISA test

SOP/CISA/ELISA/1.

Method accredited UNE-EN ISO/IEC 17025:2017.

Detection of ASFV antibodies by the commercial INGENASA blocking ELISA test (INGEZIM PPA COMPAC K3.)

SOP/CISA/ELISA/2.

Method accredited UNE-EN ISO/IEC 17025:2017.

Detection of ASFV antibodies by the WOAH-Immunoblotting (IB)

SOP/CISA/IB/1.

Method accredited UNE-EN ISO/IEC 17025:2017.

Detection of ASFV antibodies by the WOAH-immunoperoxidase test (IPT)

SOP/CISA/IPT/1.

Method accredited UNE-EN ISO/IEC 17025:2017.

ASFV isolation, titration and identification by the haemadsorbing (HAD) test in swine primary cell cultures